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BD CaliBRITE™ 3 Beads (Cat. No. 340486). Unless otherwise specified, all products are for Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for resale. 8995 37 BD™ Cytometric Bead Array Limitations Chapter 2 The BD CBA Kits provide several advantages when compared with some immunoassay methods. For example, the required sample volume for measuring multiple analytes is smaller than some conventional immunoassays wherein only one analyte can be measured per sample. Due to the BD CBA Kit’s capacity to detect six analytes in a single sample, the BD CBA Human Th1/Th2 Cytokine Kit requires approximately one-sixth the sample volume required when compared with a conventional immunoassay.

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Human PBMCs were isolated from heparinized whole blood by density centrifugation on Ficoll Paque™ and were either cultured for 4 hrs with no activators and no protein transport inhibitors (A, B) or were stimulated with PMA (5 ng/ml, Sigma, Cat. No. P-8139) and Ionomycin (500 ng, Sigma, P-8139) in the presence of either brefeldin A (BD GolgiPlug, Cat. No. 555029) (E, F), monensin (BD GolgiStop, Cat. No. 554724) (G, H) or without any protein transport inhibitor (C, D). Following incubation the cells were harvested, fixed and permeabilized with BD Cytofix/Cytoperm reagents (Cat.

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Account of the late æronautical expedition from London to Weilburg,: Accomplished by Robert Hollond, esq., Monck Mason, esq., and Charles Green, aeronaut by Monck Mason


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