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New PDF release: Advances in Applied Microbiology, Vol. 22

By D. Perlman (Ed.)

ISBN-10: 0120026228

ISBN-13: 9780120026227

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Conclusion On the basis of our experience in industrializing one immobilized enzyme system and two immobilized microbial cell systems, we consider that the utilization of immobilized microbial cells for continuous enzyme reaction has some advantages as follows. 1. Processes for extraction andlor purification of enzyme are not necessary. 2. Yield of enzyme activity on immobilization is high. 3. Operational stability of immobilized cells is generally high. 4. Cost of enzyme is low. Further, we consider that the enzyme reactions by immobilized microbial cells are promising and advantageous in the following cases: (a) when en- TRANSFORMATIONS BY IMMOBILIZED MICROBIAL CELL5 25 zymes are intracellular; (b) when enzymes extracted from the cells are unstable; (c) when enzymes are unstable during and after immobilization; (d) when the microorganism contains no interfering enzymes; (e)when interfering enzymes are readily inactivated or removed; (f) when substrates and products are not higher molecular compounds.

ISe L-Ar@nine N Hz L-Citrullme In most cases, however, a part of L-citrulline formed is further converted to L-ornithine by the action of ornithine transcarbamylase. On the other hand, we found that Pseudomonus putida has a higher activity of L-arginine deiminase and no activity of ornithine transcarbamylase. The microorganism has been industrially used for the production of L-citrulline at Tanabe Seiyaku Co. Ltd. , 1971). These procedures have been carried out in a batch process by incubating a mixture of substrate and fermentated broth.

Enzyme Eng. 2, 303313. , and Nabe, K. (1974a). Japanese Patent Kokai 81,590/74. , and Nabe, K. (1974b). Japanese Patent Kokai 81,591/74. , and Mori, T. Japanese Patent Kokai 30,582/74. , and Sato, T. (1974d). Japanese Patent Kokai 132,290/74. , and Sato, T. Appl. Microbiol. 27, 878885. , and Uchida, T. (1975a). Japanese Patent Kokai 135,290/75. , and Yamamoto, K. (1975b). Japanese Patent Kokai 100,289/75. , and Yamamoto, K. Enzyme Eng. 3 (in press). Dinelli, D. (1972). Process Biochem. August 9-12.

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Advances in Applied Microbiology, Vol. 22 by D. Perlman (Ed.)


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