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Download PDF by Xuejun Gu, Bryan J. Harmon, Daniel I. C. Wang (auth.),: Advances in Bioprocess Engineering: Volume II

By Xuejun Gu, Bryan J. Harmon, Daniel I. C. Wang (auth.), Enrique Galindo, Octavio T. Ramírez (eds.)

ISBN-10: 9048149878

ISBN-13: 9789048149872

ISBN-10: 9401706433

ISBN-13: 9789401706438

Bioprocess engineering has performed a key function in biotechnology, contributing in the direction of bringing the fascinating new discoveries of molecular and mobile biology into the utilized sphere, and in preserving proven techniques, a few centuries-old, effective and crucial for contemporary undefined. Novel advancements and new program parts of biotechnology, in addition to expanding constraints in expenditures, product caliber, regulatory and environmental concerns, have positioned the biochemical engineer on the leading edge of latest demanding situations. This moment quantity of Advances in Bioprocess Engineering displays exactly the multidisciplinary nature of the sphere, the place new and conventional parts of program are nurtured via a greater figuring out of primary phenomena and by means of the usage of novel ideas and methodologies. The chapters during this booklet have been written by way of the invited audio system to the 2d overseas Symposium on Bioprocess Engineering, Mazatlan, Mexico, September 1997.

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E. over a month [61]) possibly due to the appearance of defective virus after excessive successive passages in the infection vessel. Defective virus lack up to 40% of the viral genome, including the gene of interest and genes required for viral replication, and thus require intact virus for their propagation [138]. Defective virus also replicate faster than complete virus and compete with intact virus for cellular proteins and enzymes [129, 138]. Defective virus can be detected after passage 10, and their negative effect on protein production is very severe after passage 20 [61].

RAMIREZ recombinant virus, its safety for humans and mammals, possibility of simultaneous expression of two or more proteins, and its high versatility. The increasing interest placed in the IC-BEVS can be seen in Figure 1, where the number of cites appearing in the Derwent Biotechnology Abstracts database related to protein production in insect cell culture is shown. As seen, the number of references increased exponentially from 1988 to 1991. After a small decrease in 1992, reports have continued to increase until 1995.

At 20 hours post infection (bpi), polyhedra occluded virus begin to appear (very late infection phase). Cell lysis occurs from 72 bpi. For recombinant protein production, the foreign DNA is usually positioned in place of polyhedrin, which is not required for viral protection in vitro. The promoter of polyhedrin is very strong: after infection with wild type baculovirus, polyhedrin can constitute up to 50% of the cellular protein. I! c Late infection phase 12·20 hpi I ~econdary infection Budded virus Primary infection Budded virus l T ~ ~ c a)~~ Occluded virus Figure 2.

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Advances in Bioprocess Engineering: Volume II by Xuejun Gu, Bryan J. Harmon, Daniel I. C. Wang (auth.), Enrique Galindo, Octavio T. Ramírez (eds.)


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