By Thomas M. Bell (Auth.)
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Many compounds, both natural and synthetic, will inhibit viral multiplication in vitro but almost without exception they have little or no effect in the intact host when administered in doses which are not toxic. The naturally occurring compounds are similar to the antibacterial antibiotics, while the synthetic compounds are analogues of such essential metabolites as purine and pyrimidene bases and amino acids. The main problem is that most, if not all, of these inhibit the metabolism of normal cells as well as that of infected cells.
1947) Brit. J. Exper. Path. 28, 278. 9. , Rosenberg, E. B. (1947) /. Exper. Med. 86, 423. 10. Cantell, K. (1961) Advances in Virus Research, 8, 123. 11. , Burke, D. C. (1959) Brit. Med. Bull. 15, 185. 12. Burke, D. C. (1961) Biochem. J. 78, 556. 13. , Cantell, K. (1962) Virology, 18, 145. 14. Wagner, R. R. (1961) Virology, 13, 323. 15. Ho, M. (1961) Proc. Soc. Exp. Biol. Med. 107, 639. 16. , Schonne, E. (1962) Virology, 16, 63. 17. , Enders, J. F. (1959) Virology, 9, 446. 18. Chany, C. (1960) Compt.
If the detection system involves an all or nothing phenomenon like the death of the host or CPE in a tube culture, excess 38 AN INTRODUCTION TO GENERAL VIROLOGY antibody must be present to neutralise the new infectious virus produced by the few infectious particles left. This can be overcome in practice by using very small virus inocula such as 100 TCD 50 so that all the virus is neutralised. 1 The Neutralisation Tests in routine use number seven. The mouse protection test as devised by Sawyer & Lloyd6 is used for the Arboviruses and Coxsackie A viruses.
An Introduction to General Virology by Thomas M. Bell (Auth.)